Abstract: OBJECTIVE To evaluate the effect of liquefaction,inoculation and the growth of bacteria of Digest-EUR（BioMerieux）,SPUTASOL（OXOID）and trypsin（Sigma）sputum digestive reagents with the Previ Isola automatic flat inoculation system（ISOLA）,providing a basis for the selection of sputum digestion reagents.METHODS120 of moderate viscous sputum specimens and severe viscous sputum samples were collected in PLA General Hospital inpatients,from October 2014 to January 2015,which were digested by three above reagents,to assess the efficacy of inoculation.Three reagents respectively processed Streptococcus pneumonia,Haemophilus influenzae bacteria at 0min,15 min,30min,60 min,90min,120 min.After that,the growth of bacteria was observed.SPSS statistical software statistical analysis was performed.RESULTS After three reagents for both of viscous sputum samples digestion process,the ISOLA tips were not clogged.The digestion pretreatment effect was no significantly different.There was no significant difference effect on three kinds of Colombia blood plates,Chinese blue plates and chocolate plates.Digest-EUR and SPUTASOL had no effect on the growth of S.pneumoniae,H.influenzae bacteria at different times,while trypsin suppressed S.pneumoniae and H.influenzae growth at 15 min.CONCLUSION The three reagents are available for pre-digested sputum specimens with ISOLA.Digest-EUR and SPUTASOL have no significant effect on the growth of S.pneumoniae and H.influenzae.Howere,trypsin liquefaction after 15 min inhibits S.pneumoniae and H.influenzae growth.