Abstract: OBJECTIVE To investigate the distribution of mobile resistant determinants (genetic markers of mobile genetic elements and resistant genes) in a group of drug-resistant Escherichia coli (DR-ECO), so as to provide evidence to clinical treatment. METHODS From Jan. to Dec. 2015, a total of 32 strains of DR-ECO isolated from inpatients sputum were collected from the hospital. Then, 3 kinds of mobile genetic elements, 16 kinds of beta-lactamase genes were analyzed by polymerase chain reaction (PCR) and sequence analysis method. Finally, relationships among strains were performed by sample cluster analysis (UPGMA). RESULTS In 32 strains of DR-ECO, a kind of mobile genetic element was detected, which was intⅠ1 27 strains(84.4%);general positive rates of β-lactamase drug-resistant genes were 100.0%,and 4 kinds of positive β-lactamase genes were:blaTEM, blaSHV, blaCTX-M-1 cluster and blaOXA-1 cluster. General positive rates of resistant genes to aminoglycosides was 93.8%,and 5 kinds of positive genes were:aac(3)-Ⅱ, aac(6')-Ⅰb, ant(3″)-Ⅰ, ant(2″)-Ⅰ and aph(3')-Ⅰ. CONCLUSION Mobile resistant determinants detected from this group of DR-ECO play a key role in resistance to beta-lactams, aminoglycosides, sulfamethoxazole/trimethoprim, furthermore, the phenotypes and genotypes were coincided. Three different clones in this group suggested nosocomial infection in the hospital.