Abstract: OBJECTIVE To investigate drug resistance of clinical isolates of Candida to fluconazole in the presence of ibuprofen, and confirm the reversal effect of ibuprofen on clinical isolates of Candida resistance to fluconazole. METHODS A total of 308 strains of Candida were isolated from our hospital from Feb. 2014 to Feb. 2016. Susceptibility test was determined by ATB Fungus 3 strip, and the changes of MICs of Candida to fluconazole in the presence of ibuprofen were measured. RESULTS Sixty-two of the 308 strains isolated were resistant to fluconazole. The strains of C. albicans, C. tropicalis, C. glabrata, C. parapsilosis and C. krusei were 2, 30, 4, 18 and 8, respectively. Among these isolates, 35 strains(56.45%)demonstrated reversal of fluconazole resistance with concomitant use of ibuprofen. The reverse rates of C. albicans, C. tropicalis, C. parapsilosis, C. krusei and C. glabrata were 100%, 53.33%, 83.33%, 25.00%, and 0, respectively. The differences were significant(P＜0.05). CONCLUSION Our findings prove the reversal of fluconazole resistance of Candida with concomitant use of ibuprofen and suggest a potential therapeutic alternative that can be useful in fluconazole-resistant clinical isolates of Candida.