Abstract: OBJECTIVE To explore the supervision and inspection mechanism of hospital-acquired infection management of maternal and child health hospital, in order to promote continuous quality improvement of hospital-acquired infection control. METHODS The flying inspections were carried out once a month in 2015, with one group a time, and reviewed it next month, which shared the same content and methods. The results of the each two inspections were compared to understand whether the effect of continuous quality improvement could be achieved through flying inspections and subsequent work. RESULTS Compared with the first inspection, the review inspection of the hand hygiene compliance, hand-washing correctness, the awareness rate of hospital-acquired infection, the sampling qualified rate of body surface and hands in working condition increased, with significant differences (P＜0.05). CONCLUSION Long-term mechanism of flying inspection combined with hospital grade assessment should be established to facilitate the continuous quality improvement of hospital-acquired infection management.