Abstract: OBJECTIVE To understand the current status of bacterial contamination of personal digital assistants (PDA) and evaluate the effects of interventions so as to provide guidance for standard hygiene management of mobile devices. METHODS The onsite hygienic sampling was carried out for the PDA that were in use in clinical departments and the quantitative bacterial test was conducted, the samples that were collected before the PDA cleaning and disinfection management system was implemented were assigned as the experimental group, while the samples that were collected after the system was implemented were set as the control group.The status of isolation of bacteria was observed and compared between the two groups before and after the interventions were taken. RESULTS A total of 167 sets of PDA were sampled, including 112 sets from the experimental group and 55 sets from the control group; the bacteria were isolated from 30 sets in the experimental group, with the positive rate 26.79%, and the bacteria were isolated from 5 sets in the control group, with the positive rate 7.27%; there was significant difference in the positive rate of detection of bacteria between the two groups (χ²=8.66,P＜0.01).Totally 34 strains of pathogens were isolated, among which the gram-positive bacteria were dominant; Bacillus subtilis, Staphylococcus epidermidis, and Enterococcus faecium ranked the top 3 species of pathogens, accounting for 35.29%, 20.59%, and 20.59%, respectively. CONCLUSION The PDA in use tend to be contaminated by bacteria.It is necessary to carry out regular cleaning and disinfection and strengthen the hand hygiene management so as to prevent the PDA from being a novel transmission medium.