Abstract: OBJECTIVE To understand the homology of pandrug-resistant Acinetobacter baumannii causing nosocomial infection and the disinfectant resistance gene qacEΔ1 so as to put forward targeted prevention measures and effectively prevent the nosocomial infection. METHODS Totally 15 strains of pandrug-resistant A.baumannii were isolated from the hospitalized patients in 2014, then the homology was analyzed by using Enterobacteriaceae repetitive intergenic sequences-polymerase chain reaction (ERIC-PCR), and the disinfectant resistance gene qacEΔ1 was detected with the use of polymerase-chain-reaction(PCR). RESULTS The 15 strains of pandrug-resistant A.baumannii were divided into the genotype A (10 strains) and the genotype B (5 strains) through ERIC-PCR. Totally 6 strains of genotype A and 3 strains of genotype B were isolated from ICUs, the rest of 4 strains of genotype A respectively distributed in the PICU (pediatric ICU), plastic surgery wards, cardiovascular surgery wards, and neurosurgery wards. The rest of 2 strains of genotype B distributed in the gastrointestinal surgery wards and bone surgery wards. All of the 15 strains of pandrug-resistant A.baumannii causing nosocomial infection carried with the disinfectant resistance gene qacEΔ1. CONCLUSION The genotype A/B pandrug-resistant A.baumannii is prevalent in the hospital and mainly distributes in the ICUs where the underlying diseases are severe and the invasive operations are frequent. The carrying rate of the disinfectant resistance gene qacEΔ1 is extremely high in the pandrug-resistant A.baumannii causing nosocomial infection.