Abstract: OBJECTIVE To explore the drug resistance, distribution, and molecular epidemiological characteristics of Acinetobacter baumannii strains in tertiary general hospitals so as to provide guidance for long-term surveillance and prevention of outbreak and prevalence. METHODS A total of 683 strains of A.baumannii were isolated from 3 tertiary general hospitals from Jan 2014 to Dec 2014, then the drug resistance rates were detected by using disk diffusion method, and the statistical analysis was performed with the use of WHONET5.5 software.Totally 27 strains of imipenem-resistant or meropenem-resistant A.baumannii were randomly selected for the detection of genes, and the homology of the strains was analyzed by means of ERIC-PCR. RESULTS The isolation rate of A.baumannii strains was 5.50%, and the isolation rate of multidrug-resistant A.baumannii (MDRAB) strains was 63.10%.The drug resistance rate to piperacillin was the highest (62.08%), the drug resistance rate to minocycline was the lowest (21.08%), and the drug resistance rate to cefoperazone-sulbactam was 37.04%.The major carbapenemase genes were as follows: OXA-23, OXA-24, OXA-51, OXA-64; there were 4 clones which distributed in 3 hospitals, and the type A was the most prevalent. CONCLUSION The isolation rate of MDRAB strains is high in Binzhou area; the strains are highly drug-resistant, indicating that cefoperazone-sulbactam and minocycline can be carefully chosen for empirical medication for the MDRAB infection.The detection of OXA-51 may facilitate the rapid identification of the A.baumannii strains.The different types of A.baumannii strains scatter in the hospitals, and the same clone strains are prevalent and spread among the hospitals, which should be attached great importance to.