Abstract: OBJECTIVE To investigate the active screening of adhesion toxin and superantigen genes in Staphylococcus aureus isolated from ICU patients so as to provide guidance for development of prevention and control programs. METHODS From 2013 to 2014, a total of 46 strains of S.aureus that were isolated from nasal swab and given active screening were collected, then the strains were identified by using chromID S.aureus plate sreening and Vitek-2 bacterial identification system of BioMérieux, France, and 16 kinds of adhesion toxin genes and 20 kinds of superantigen genes were detected with the use of polymerase-chain-reaction (PCR). RESULTS Totally 9 kinds of adhesion toxin genes and 10 kinds of superantigen genes were detected in the 46 strains of S.aureus; the total detection rate of the adhesion toxin genes was 100.0%, and total detection rate of the superantigen genes was 65.0%. CONCLUSION The high detection rate of the adhesion toxin genes and superantigen genes in the S.aureus is the leading cause of pulmonary or bloodstream infection in the ICU patients.