Abstract: OBJECTIVE To evaluate the results of original identification, Vitek MS and Bruker MS identification of Candida glabrata species complex collected from National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) in 2010-2014. METHODS A total of one C. bracarensis isolate and twelve C. nivariensis isolates were collected from the CHIF-NET in 2010-2014, and 411 C.glabrata isolates were collected from eleven hospitals which participated in the CHIF-NET in five consecutive years from 2010 to 2014. All of the strains were identified by using molecular method, the review identification results were set as "gold-standard", the results of original identification of the conventional methods were evaluated, and the performances of Vitek MS and Bruker MS matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) in the identification of the C.glabrata species complex were observed. RESULTS As compared with the molecular identification, the accurate rate of original identification of C.glabrata was 90.3%, and 9.7% of the strains were mistakenly identified as Candida parapsilosis and Candida albicans by CHROMagar chromogenic media, ID 32C, API 20C or Vitek 2 Compact system. C.nivariensise and C.bracarensis could not be identified by using routine identification methods.The accurate rates of the Vitek MS and Bruker MS were 97.6% and 100.0%, respectively.There were no reference spectra for C.bracarensis and C.nivariensis in the Vitek MS 2.0 database, so the two species could not be identified.There were reference spectra for the two species in the Bruker Biotyper 3.1 database, however, one C.bracarensis isolate could not be identified and all of the 12 C.nivariensise isolates were identified correctly, with the spectral score varying from 1.708 to 1.944. CONCLUSION It is for the first time to evaluate the performances of Vitek MS and Bruker MS in identification of the C.glabrata species complex in China and is concluded that the two methods are reliable for the identification of the C.glabrata. Bruker MS is superior to Vitek MS in identification of the rare species of Candida.