Abstract: OBJECTIVE To explore the correlation between the quinolones resistance phenotypes of Klebsiella pneumoniae and PMQR genes so as to provide theoretical basis for control of prevalence of multidrug-resistant Klebsiella pneumoniae . METHODS A total of 90 clinical K.pneumoniae isolates were collected from Jan 2014 to Dec 2015, including 30 strains susceptible to ciprofloxacin, 20 strains intermediate, and 40 strains resistant.The PMQR genes and insert sequence ISCR1 were detected by using PCR, the PMQR genes in the insert sequence downstream of the ISCR1-positive strains were detected by means of PCR-mapping, and all of the PCR products were determined through DNA sequencing. RESULTS Of the 30 strains of K.pneumoniae susceptible to ciprofloxacin, 8 (26.67%) were tested positive for qnrA gene, and the mobile elements were not detected.Of the 20 strains of K.pneumoniae intermediate to ciprofloxacin, 17 (85.00%) strains were positive for qnrA, 7 (35.00%) strains were positive for qnrB, 5 (25.00%) strains were positive for qnrS, and 11 (55.00%) strains were positive for acc(6')-Ib-cr gene; totally 15 (75.00%) strains were positive for ISCR1.The results of PCR-mapping showed that the ISCR1-qnrA was detected positive in 14 (70.00%) strains, ISCR1-qnrB was detected positive in 6 (30.0%) strains, ISCR1-qnrS was detected positive in 5 (25.00%) strains, and ISCR1-acc(6')-Ib-cr was detected positive in 4 (20.00%) strains.Among the 40 strains of multidrug-resistant K.pneumoniae , 33 (82.50%) strains were tested positive for qnrA, 12 (30.00%) strains were tested positive for qnrB, 16 (40.00%) strains were tested positive for qnrS, and 28 (70.00%) strains were tested positive for acc(6')-Ib-cr 28. Totally 30 (75.00%) strains were tested positive for ISCRI gene, and the 30 strains were tested positive for ISCR1-qnrA1, with the positive rate 75.00%; the positive rates of ISCR1-qnrB4 and ISCR1-qnrS1 were 37.50% and 30.00%, respectively. CONCLUSION The carrier rate of the PMQR genes in the quinolones-intermediate K.pneumoniae strains is not as low as that in the quinolones-resistant K.pneumoniae strains, the strains can transmit through the mobile elements, therefore, it is necessary to pay attention to the control of the multidrug-resistant strains.