Abstract: OBJECTIVE To evaluate the effects of comprehensive interventions on prevention and control of nosocomial infections so as to effective reduce the incidence of the nosocomial infections. METHODS A total of 133 482 patients who were hospitalized from 2011 to 2015 were recruited as the study objects.The interventions included establishing management platform, boosting the early warning information system of nosocomial infections, carrying out the comprehensive evaluation of health care, and strengthening the management of nosocomial infections in high-risk departments.The incidence of nosocomial infections, incidence of type Ⅰ incision infection, incidence of MDROs infections, and hand hygiene compliance rate were observed and compared before and after the intervention. RESULTS The incidence of nosocomial infections was remarkably reduced after the interventions were taken, dropping from 1.80% to 1.06%, and there was significant difference (P＜0.01).The incidence of type Ⅰ incision infections was decreased in the general surgery department, orthopedics department, and other surgery departments; the incidence of type Ⅰ incision infections dropped from 0.33% to 0.23% in the whole hospital.The incidence of MDROs infections was significantly decreased from 0.48‰ to 0.24‰.The hand hygiene compliance rate was increased year by year, rising from 38.2% to 87.2%. CONCLUSION The comprehensive interventions can effectively reduce the incidence of the nosocomial infections.