Abstract: OBJECTIVE To investigate the economic burden due to multidrug-resistant organisms (MDROs) infections in hospitalized patients so as to provide guidance for reduction of incidence of the MDROs infections and economic burden. METHODS By means of case-control study, a total of 108 patients who were hospitalized from Jan 1, 2014 to Dec 31, 2014 were enrolled in the study, 54 patients with MDROs infections were assigned as the infection group, and 54 patients without nosocomial infections were set as the control group.The length of hospital stay and hospitalization costs were observed and compared between the two groups. RESULTS The average length of hospital stay was 39.3 days in the infection group, 19.6 days in the non-infection group, with the difference value 19.7 days, and there was significant difference (P＜0.05).The average hospitalization costs were 160 807.6 yuan in the infection group, 67 543.4 yuan in the non-infection group, the difference value of the total hospitalization costs was 93 264.2 yuan, and there was significant difference (P＜0.05). CONCLUSION The average length of hospital stay of the hospitalized patients with MDROs infections is significantly longer than that of the hospitalized patients without nosocomial infections; the hospitalization costs of the hospitalized patients with MDROs infections are significantly more than those of the hospitalized patients without nosocomial infections.It is necessary to take effective measures so as to reduce the incidence of MDROs infections, cut the hospitalization costs, and accelerate the turnover of beds.