Abstract: OBJECTIVE To prospectively study the risk relationship between four kinds of routine testing of liver enzymes and HBV-related liver cancer, so as to provide a reference for the clinical diagnosis. METHODS Clinical data of 294 HBV patients treated in our hospital from Jun. 2008 to Jun. 2013 were collected. The relationship of four kinds of routine testing of liver enzymes (alanine aminotransferaseALT, aspartate aminotransferaseAST, alkaline phosphataseALP, and γ-glutamine GGTGGT) with clinicopathological characteristics of patients were evaluated. The predictive values of all four kinds of liver enzymes in HBV-related liver cancer were analyzed by Kaplan-Meier and logistic methods. RESULTS In 294 patients, a total of 26 patients developed into liver cancer at the end of follow-up, with the incidence of 8.8%. In four kinds of liver enzymes, GGT and ALP were independent risk factors for HBV-related liver cancer. Prediction model (AG) based on ALP and GGT could effectively predict the risks of liver cancer in patients with hepatitis B. CONCLUSION Serum GGT and ALP are predictors of the occurrence of hepatitis B patients with liver cancer.