Abstract: OBJECTIVE To investigate the values of dynamic monitoring of C-reactive protein, procalcitonin, white blood cell count and pro-adrenomedullin in predicting nosocomial infection of children patients in PICU. METHODS From Dec. 2014 to Dec. 2015, 62 children patients with clinical manifestations of infection were selected in PICU of our hospital. Totally 26 children patients were met the diagnostic criteria of nosocomial infection as infection group, and 36 children patients were not met the diagnostic criteria of nosocomial infection as high-risk group. During the same period, 50 children patients with no symptoms of infection were selected as control group. The clinical indexes of the children patients in the three groups were compared and analyzed. RESULTS After 48h and 96h in PICU, compared with control group, the levels of CRP, PCT, WBC and pro-ADM of children patients in infection group and high-risk group were increased significantly (P＜0.05). CONCLUSION After 48h in PICU, the children patients have the higher risk for hospital infection. Combined detection of CRP, PCT, WBC and pro-ADM levels has high reference values for the prediction of hospital infection.