Abstract: OBJECTIVE To evaluate the role of culture construction of nosocomial infection management in control of nosocomial infections through review of cultural construction of infection control. METHODS The working vision of ' hospital-wide collaboration, joint efforts for control of infection' was gradually established through the review of historical inheritance of infection control, the signature sign of nosocomial infection was designed, the work targets and policies were set up, Tiantan Newsletter and Bulletin of Infection Control were launched to create a brand of "Tiantan infection control", and the culture of lead, incentive and service was energetically advocated during the work of infection control. RESULTS A brand of infection control and cultural connotations was established through the activities such as ' Tiantan Infection Control Week' and ' selection of star of infection control'.Gantt chart was used for work management and the infection control knowledge was popularized through the network course.As for working methods, the multicultural methods were introduced to propaganda infection control through the history of hospital infection, thematic philately, and photography.The communication among departments was conducted through the web page, public e-mail, QR code.The road of specialization of nosocomial infection management was explored so as to make the infection control problems come from the hospital and serve the hospital. CONCLUSION The establishment of culture of nosocomial infections has improved the influence and execution force of nosocomial infection control, and the entire staffs' recognition of importance of infection control has been improved through rich cultural activities.