Abstract: OBJECTIVE To understand the clinical distribution characteristics and changes of drug resistance of Acinetobacter baumannii in non-sputum specimens of our hospital during 2013-2014. METHODS Totally 239 strains of pathogens isolated from non-sputum specimens were collected from Jan. 2013 to Dec. 2014 in the hospital. Automation instrument method and broth dilution method were used for the drug sensitive test, and the results were statistically analyzed with the SPSS22.0 software. RESULTS A.baumannii mainly distributed in the ICU, accounting for 46.86%. The highest detection rate was blood specimen,accounting for 32.64%. The drug resistant rates to the most commonly used antibiotics were more than 50%, and there was an increasing trend year by year(P＜0.05). Resistant A.baumannii had higher sensitivities to tigecycline and minocycline. CONCLUSION The resistant rate of A.baumannii in non-sputum specimens to commonly used antimicrobial agents in hospital has an increasing trend year by year, and resistant A.baumannii has higher sensitivity to tigecycline and minocycline, which can be used as empirical drug candidates.