Abstract: OBJECTIVE To investigate the antimicrobial resistance and virulence genotypes epidemiology of hypermucoviscous Klebsiella pneumoniae(hvKP) isolates in the liver abscess disease, so as to procide evidence for clinical treatment. METHODS One hundred and twelve strains of hvKP were isolated from puncture fluid and blood separation of patients with diagnosis of the liver abscess in our hospital from Jan. 2011 to Dec. 2015. The major virulence genotypes in the hvKP isolates were detected by PCR. RESULTS All the 112 strains of hvKP were hypervirulent isolates by the string test. There were 26 ESBLs positive strains, with the positive rate of 23.2%. Except imipenem and meropenem, the antimicrobial resistant rates of ESBLs positive strains to antimicrobial agents were significantly higher than those of ESBLs negative strains (P<0.05). Virulence genes were detected from 91.1%(102/112) of the isolates , and were not detected from 10 isolates. The K1+rmpA+wcaG (33.3%, 34/102) acted as the predominant virulence genotypes carrying mode. CONCLUSION K.pneumoniae strains isolated from the liver abscess are all hypervirulent isolates. The rmpA, wcaG and K1 are the major genotypes carried by the hvKP from our hospital, and K1 plus rmpA plus wcaG (K1+rmpA+wcaG) is the predominant carrying mode of the virulence genotypes.