Abstract: OBJECTIVE To understand the clinical distribution and drug resistance of Pseudomonas aeruginosa, and provide reliable basis for the infection control of P.aeruginosa in hospital. METHODS A total of 8078 strains of P.aeruginosa were collected from Jan. 2010 to Dec. 2015. Identification and antibiotic susceptibility tests of the strains were carries out by VITEK-2 compact automatic susceptibility identification analyzer. The antibiotic susceptibility results were analyzed by WHONET5.6. RESULTS Among all isolated gram-negative bacteria, the isolation rate of P. aeruginosa was 27.59%. The majority of clinical isolates originated from sputum samples,accounting for 79.57%.Infection departments were mainly ICU and department of respiration, with the isolation rates of 24.33% and 16.86% respectively. Drug susceptibility tests results showed that the drug resistant rates of P. aeruginosa were higher to most of the beta-lactam drugs, quinolones and aminoglycoside class drugs, was the lowest to amikacin, and had a decreasing trend to ceftazidime. The overall detection rate of multi-drug resistant strains was 71.17%. CONCLUSION The drug resistance of P. aeruginosa is serious. The hospital infection monitoring system should be established and improved, and the doctors should use the antibiotics more reasonably and scientifically, timely adjustment of antibiotics, to avoid the abuse of antibiotics.