Abstract: OBJECTIV Healthcare-associated infection is not only a public health issue but also a serious clinical problem. The incidence of healthcare-associated infection may lead to the increase of morbidity rate and mortality rate of patients as well as the medical cost. The healthcare-associated infection is usually induced by the pathogens that are resistant to antibiotics, however, the surfaces of objects in hospital environment and hands of healthcare workers may become the carriers of pathogenic microorganisms and form a cross chain for transmission of infection, which is closely associated with the incidence of healthcare-associated infection. Therefore, it is the key link to choose appropriate disinfectant for effective eradication of pathogenic microorganisms so as to prevent and control the healthcare-associated infection, the pathogens' carrying with resistance genes to disinfectants and emergence of resistance to the commonly used disinfectants are most concerned. This paper reviews the present situation of the resistance of the pathogens to antibiotics and disinfectants.