Abstract: OBJECTIVE To investigate the correlation between epstein-barr virus(EBV) infections and classification in non-hodgkin lymphoma(NHL) of pharyngeal and nasal cavity, so as to provide references for clinical diagnosis and treatment of NHL. METHODS Lesion tissue specimens of 127 cases of patients with pharyngeal and nasal cavity NHL from Mar. 2015 to Mar. 2016 were selected. Immunohistochemical staining SP method and in situ hybridization of EBVR-1 were carried out, and the results were analyzed. RESULTS There were 86 cases of NK/T cell lymphoma, 31 cases of B cell lymphoma, and 10 cases of T cell lymphoma, accounting for 67.72%, 24.41%, and 7.87%, respectively. Nasal cavity and pharyngeal NHL patients were mainly NK/T cell lymphoma, accounting for 81.58% and 66.67%. Amygdala NHL patients were mainly B cell lymphoma, was accounting for 62.50%. There were significant differences in NK / T-cell lymphoma and B-cell lymphoma classification between the above lesions (P<0.05). Totally 85 cases were EBVR-1 positive of NK/T cell lymphoma, 14 cases were EBVR-1 positive of B cell lymphoma, and 7 cases were EBVR-1 positive of T cell lymphoma, with the positive rates of 98.84%, 45.16% and 70.00%. The positive rates of EBVR-1 in different lymphoma classification was significant (P<0.05). CONCLUSION NK/T cell lymphoma is common in pharyngeal and nasal cavity NHL classification, but B cell lymphoma is common in amygdala NHL classification, and EBV infection may play an important role in the pathogenesis and development of NK/T cell lymphoma.