Abstract: OBJECTIVE To study the drug resistant genes of carbapenem-resistant Acinetobacter baumannii (CRAB) producing serine proteases (class A enzyme), oxacillin hydrolases (class D enzyme) and metalloenzymes, so as to provide references for the treatment and control of CRAB infection. METHODS Identification and drug susceptibility analysis of 57 cases of CRAB strains from Jan. 2011 to Dec. 2015 were carried out by automatic microbial analysis system VITEK-2. Resistant genes of blaOXA-51, blaOXA-23, blaOXA-24, blaOXA-58, blaPER-1, blaVIM, and blaIMP were detected by polymerase chain reaction (PCR). RESULTS Of the 57 strains of CRAB, 34 (59.5%) were isolated from ICUs, and 11 (19.3%) were isolated from neurosurgery departments; 33 (58.0%) were isolated from blood specimens, and 12 (21.0%) were isolated from cerebrospinal fluid specimens. The drug susceptibility rate of the CRAB strains to sulfamethoxazole-trimethoprim was as high as 63.6%, the drug susceptibility rates to the rest of drugs were less than 40.0%; the drug resistance rates to cephalosporins and carbapenems were 100.0%. The detection rates of blaOXA-51, blaOXA-23 and blaOXA-24 of the 57 CRAB strains were 100.0%, 96.5% and 12.3%, respectively, the detection rate of blaPER-1 was 5.3%, among them 5 strains simultaneously carried blaOXA-23 and blaOXA-24, 3 strains simultaneously carried blaOXA-23 and blaPER-1, and no blaOXA-58, blaVIM and blaIMP strains were found. CONCLUSION The CRAB resistant gene in this region is mainly blaOXA-23, some strains carried blaOXA-24 and blaPER-1, the joint resistance of different resistant genes has appeared, and no metallogenase gene was found.