Abstract: OBJECTIVE To investigate the clinical influence of prevention effect and immunological parameters of mannatide injection on breast surgery incision infections, so as to provide references for clinical prevention of breast surgery incision infections. METHODS A total of 132 patients of breast surgery admitted to the hospital from Jan. 2015 to Aug. 2015 were chosen. According to the treatment methods, they were divided into study group (with mannatide injection) and control group (normal saline), with 66 cases in each group. In study group, mannatide injection was administered by intravenous infusion in physiological saline at a dose of 10 mg / day from 1 h before the surgery to 1 week after the surgery, and patients in control group were received only the same dose of normal saline. The incision infection rate, immunological indexes of CD₃⁺, CD₄⁺, CD₄⁺/CD₈⁺, and NK, and immune function indexes complements C₃ and C₄, as well as hospital stay and hospitalization expenses were observed and compared between the two groups. RESULTS The incision infection rate of study group was 0.00%, which was lower than 6.06% of control group. The CD₃⁺, CD₄⁺, CD₄⁺/CD₈⁺, and NK of study group were (81.34±9.34)%, (50.42±7.43)%, (1.92±0.84)% and (27.34±9.11)% after surgery, which were higher than those of control group of (66.32±9.51)%, (41.90±5.43)%, (1.43±0.76)%, and (19.34±7.43)%. The C₃ and C₄ levels after surgery in study group were (1.05±0.46) g/L and (0.50±0.22)g/L, which were higher than those of control group of (0.91±0.21) g/L and (0.38±0.15) g/L, and the differences were with significant (P<0.05). CONCLUSION In the clinical practice for the treatment of patients with breast surgery, the mannatide injection can effectively prevent postoperative infection in patients, improve the body's immunity levels in patients, shorter hospital stays and save the cost of treatment.