Abstract: OBJECTIVE To investigate of bacterial contamination on object surfaec in ICUs, so as to put forward intervention measures for environmental cleaning and hygiene in ICU and prevention and control of hospital infection. METHODS From Jul. 2015 to Mar. 2016, 102 samples were collected from 5 ICUs in our hospital using cotton swab method, and all specimens were determined the average number of colonies. Then the bacteria isolated from the samples with the average number of colonies exceeded were used for the identification and drug susceptibility test. RESULTS There were 70 specimens of object surfaces had the average number of colonies exceeded from 102 samples after 48 hours of culture (＞5.0 CFU/cm²), with the overall qualified rate of 31.37%. A total of 74 pathogens were isolated from 70 specimens, which mainly were 40 strains of gram-negative bacteria, accounting for 54.05%. Drug susceptibility results showed that the susceptibility of Acinetobacter baumannii to polymyxin B was 100%. CONCLUSION The environmental surfaces in ICUs were heavily contaminated by bacteria. It is necessary to further optimize and develop a more stringent environmental cleaning and disinfection process, standardize the operation and hand hygiene execution of medical staff, and cut off the risks of cross-transmission of pathogens.