Abstract: OBJECTIVE To investigate the clinical value of combined detection of blood lactate, procalcitonin and N-terminal brain natriuretic peptide for prognostic evaluation in sepsis and septic shock. METHODS A total of 118 sepsis patients from Feb. 2013 to Feb. 2016 were selected in our hospital. According to the severity of the patients, they were divided into sepsis group (n=39), severe sepsis group (n=52), and septic shock group (n=27). The levels of blood lactate, procalcitonin and N-terminal brain natriuretic peptide at different time in the three groups were compared and analyzed. The levels of blood lactate, procalcitonin and N-terminal brain natriuretic peptide of survival group and death group were compared and analyzed. RESULTS Compared with sepsis group, the levels of blood lactate, procalcitonin and N-terminal brain natriuretic peptide in severe sepsis group and septic shock group at different time were increased significantly (P＜0.05). Compared with severe sepsis group, the levels of blood lactate, procalcitonin and N-terminal brain natriuretic peptide in septic shock group at different time were increased significantly (P＜0.05).In addition, compared with survival group, the levels of blood lactate, procalcitonin and N-terminal brain natriuretic peptide in death group were increased significantly (P＜0.05). CONCLUSION Combined detection of blood lactate, procalcitonin and N-terminal brain natriuretic peptide has very important clinical guiding significance for prognostic evaluation in sepsis and septic shock.