Abstract: OBJECTIVE To investigate the relationship of maternal perinatal HBV infection status and umbilical cord blood and neonatal immune status, so as to provide reference for the related study of clinical prediction of neonatal disease. METHODS A total of 88 cases of newborns in our hospital from Dec. 2012 to Dec. 2013 were chosen as the research objects. According to maternal newborn whether infected with hepatitis B virus, they were divided into infected group and control group, with 44 cases in each group. The clinical indexes in the two groups were observed and compared. RESULTS The levels of CD₃⁺ and CD₄⁺, and IgA and IgM indexes of infected group were significantly higher than those of control group. The level of CD₁₉⁺ in infected group of newborns was (9.54±4.32)%, which was significantly lower than that of control group. The rates of neonatal mortality, hepatitis B infection, lung infection and jaundice were 11.36%, 45.45%, 31.82% and 13.64%, which were significantly higher than those of control group (P＜0.05). CONCLUSION Maternal perinatal HBV infection has effects on cord blood immune factors and lymphocyte subsets. The monitoring of umbilical cord blood of newborn can help to predict the occurrence of autoimmune diseases.