Abstract: OBJECTIVE To investigate the expression and clinical significance of Th1/Th2 cell of peripheral blood in patients with cervical cancer associated with HPV infection, so as to provide references for clinical treatment. METHODS From Jan. 2013 to Jan. 2014, 50 cases of cervical cancer patients with HPV infection were prospectively collected as study group, 50 patients with HPV infection and no cervical cancer were collected as control group. The peripheral blood T cell subsets and cytokines were compared between the two groups. The association between mortality and T cell subsets, cytokines in patients with cervical cancer associated with HPV infection was simultaneous analyzed. RESULTS Compared with control group, the level of CD₄⁺ T cell and the ratio of CD₄⁺/ CD₈⁺ of patients in study group were decreased significantly (P<0.05), IL-2, IL-25 and IL-27 levels were significantly decreased (P<0.05), and IL-10 and IL-35 levels were increased significantly (P<0.05). CONCLUSION There is a certain degree of immune suppression in patients with cervical cancer associated with HPV infection, which is related to the occurrence and development of cervical cancer.