Abstract: OBJECTIVE To compare the cleaning quality effect of different cleaning methods on loaner instrumentation, explore the application of steam cleaning in loaner instrumentation cleaning process, so as to provide the basis for optimizing loaner instrumentation cleaning process for Central Sterile Supply Department, and improving the cleaning quality of loaner instrumentation. METHODS A total of 200 loaner instrumentation were selected, and were randomly divided into 2 groups, group A were cleaned by routine method, and group B were cleaned by routine cleaning combined with steam cleaning method. The cleaning qualities of loaner instrumentation were detected by visual inspection and 3MTM Clean-TraceTM ATP bioluminescence assay, and the qualified rates of cleaning quality were recorded. RESULTS The cleaning qualified rates of loaner instrumentation group A and group B by visual inspection were 95% and 100%, and by ATP bioluminescence assay were 90% and 97%. The results showed that the cleaning quality of group B had a significant difference with that of group A (P<0.01). CONCLUSION By adopting the application of routine cleaning combined with steam cleaning in loaner instrumentation cleaning process, it can optimize the loaner instrumentation cleaning process and improve the cleaning quality of loaner instrumentation.