Abstract: OBJECTIVE To explore the distribution and drug resistance of Enterococcus isolated from urine specimens. METHODS A total of 2 872 patients with urinary tract infection who were treated in the hospital from Jan 2014 to Dec 2015 were enrolled in the study. The pathogens isolated from the urine specimens were identified, the drug susceptibility testing was performed, and the drug resistance was analyzed via chi-square test or Fisher's exact test. RESULTS Totally 2872 strains of pathogens were isolated from 2872 urine specimens, including 2 053 (71.5%) strains of gram-negative bacteria, 749 (26.1%) strains of gram-positive bacteria, and 70 (2.4%) strains of fungi. The top 5 species of isolated pathogens were in turn as follows: Escherichia coli (1399 strains, 48.7%), Enterococcus faecalis (304 strains, 10.6%), Klebsiella pneumoniae (171 strains, 6.0%), Enterococcus faecium (109 strains, 3.8%), Pseudomonas aeruginosa (94 strains,3.3%). Of the isolated pathogens, 34.5% were isolated from male patients, 65.5% from female patients, 4.1% from outpatient department patients, 95.9% from hospitalized patients, 39.7% from urology department patients, 23.4% from nephrology department patients. The drug resistance rates of the E.faecium to penicillin, ampicillin, ciprofloxacin, levofloxacin, and erythromycin were more than 80%; the drug resistance rate of the E.faecalis to erythromycin was highest, while the drug resistance rates to penicillin, ampicillin, and nitrofurantoin were very low; the drug resistance rates of the E.faecalis to penicillin, ampicillin, high concentration of gentamicin, levofloxacin, erythromycin, and nitrofurantoin were significantly lower than those of the E.faecium. The Enterococcus strains that were resistant to vancomycin, teicoplanin, or linezolid were not found. CONCLUSION The E.coli and Enterococcus are the most common species of pathogens causing the urinary tract infection. The Enterococcus strains are highly drug-resistant. The different species of Enterococcus have significantly varied drug resistance. It is necessary to reasonably use antibiotics based on the results of bacterial identification and drug susceptibility testing.