Abstract: OBJECTIVE To understand the distribution characteristics and drug resistance of multidrug-resistant bacteria in hospital, especially multidrug-resistant Acinetobacter baumannii, so as to reduce the incidence of infection by many management measures. METHODS The pathogenic bacteria were identified by automatic bacteria identification instrument, and the clinical data of multidrug-resistant bacteria were analyzed. RESULTS Totally 1573 strains of multidrug-resistant bacteria were detected in the hospital from 2013 to 2015, including 325 strains of A.baumannii, of which 127 strains occurred in ICU, which was a high risk area. Drug sensitivity analysis of the 127 strains of A.baumannii showed that drug resistance situation was serious. CONCLUSION Multidrug-resistant bacteria infection is the key and difficulty of hospital infection management, ICU multidrug-resistant A.baumannii is easy to cause hospital outbreak. Through the implementation of contact isolation measures and rational use of antimicrobial agents, can effectively control of multidrug-resistant bacteria transmission and spread, and reduce the incidence of multidrug-resistant bacteria infection.