Abstract: OBJECTIVE To investigate the pathogenic bacteria culture and drug sensitivity of oral and maxillofacial space infections, so as to provide evidence for clinical prevention and treatment of oral and maxillofacial space infections. METHODS The clinical data of 204 patients with oral and maxillofacial space infections in hospital from Jan. 2013 to Sep. 2015 were collected, and the general information of the patients, the types of pathogenic bacteria and the drug sensitivity were analyzed. RESULTS The patients with odontogenic infection accounted for 66.18%, adenogenous infection accounted for 29.90%, and iatrogenic infection accounted for 3.92%. Jaw gap was the most infection site, accounting for 59.80%, and tongue gap was the lowest, which was 2.45%. In the 204 patients, the mixed infection was 48.04%. A total of 238 strains of pathogenic bacteria were cultivated, including 147 strains of gram-positive bacteria (61.76%) and 91 strains of gram-negative bacteria (38.24%), Of which 56 strains were anaerobic bacteria(23.53%). Drug sensitivity results showed that Staphylococcus aureus had higher sensitive rates to rifampicin, Streptococcus were highly sensitive to levofloxalin, Pseudomonas aeruginosa had high sensitive rates to vancomycin, Klebsiella pneumoniae had higher sensitive rates to vancomycin and imipenem, with the susceptibility rate 100.00%. and anaerobic bacteria were highly sensitive to metronidazole, imipenem and vancomycin. CONCLUSION The main pathogens of oral and maxillofacial space infections were mixed infections. It should give anti-inflammatory treatment as soon as possible after diagnosis, and choose combination of antimicrobials with anti-anaerobic drugs according to the results of pathogenic bacteria analysis. At the same time, it should take drug sensitive test, and give targeted anti-inflammatory treatment according to the results.