Abstract: OBJECTIVE To investigate the distribution and antimicrobial resistance of pathogenic bacteria in blood culture,so as to provide a basis for the rational use of antibiotics in clinic. METHODS The distribution and the results of antimicrobial susceptibility testing and characteristics of the pathogenic bacteria isolated from blood culture samples from Jan. 2009 to Dec. 2015 in our hospital were retrospectively analyzed. WHONET 5.6 was applied for analysis. RESULTS The results showed that 2 372 pathogens were isolated from 35 368 blood culture samples from Jan. 2009 to Dec. 2015 in our hospital, and the positive rate was 6.72%, including 1 341 (56.46%) strains of gram-negative bacteria, 905 (38.11%) strains of gram-positive bacteria, and 104(4.38%) strains of fungi. Imipenem-resistant strains were found in Escherichia coil and Klebsiella pneumonia. The antimicrobial susceptibilities of Staphylococci to linezolid, vancimycin and quinupristin /dalfopristin were highly sensitive. The antimicrobial resistant rates of E. faecalis strains were much higher than those of E. faecium. CONCLUSION Gram-negative bacteria are the main pathogens of blood culture in our hospital and cabapenem-resistant strains were appeared in Enterobacteriaceae. Monitoring the changes of pathogens and trends of antimicrobial resistance was very important to guide clinical medication.