Abstract: OBJECTIVE To prospective investigation of HBV infection and five quantitative hepatitis B results of pregnant women in Zunyi City, so as to provide a reference for the prevention of HBV in mother-to-child transmission. METHODS A total of 2648 cases of pregnant women with prenatal examination from Jan. 2014 to Jun. 2016 were selected. HBsAg was detected by gold standard method, five quantitative hepatitis B results were detected by time-resolved fluorescence immunoassay technology, and HBV-DNA was detected by fluorescence quantitative PCR method. RESULTS Totally 2648 cases of pregnant women occurred 243 HBsAg positive cases, with the HBV infection rate of 9.18%. There were 134 cases of positive and 109 cases of negative HBV-DNA in HBsAg positive pregnant women, and the constituent ratio of HBV-DNA positive pregnant women was 55.14%. The order of HBV infection pregnant women were: HBsAg＋HbeAb＋HBcAb, HBsAg＋HBcAb, HBsAg＋HBeAg＋HBcAb, and pure HBsAg positive, accounting for 30.45%, 25.93%, 17.28% and 4.94%, respectively. HBV-DNA were positive in 42 cases of HBsAg＋HBeAg＋HBcAb positive pregnant women, with the positive rate of 100.00%, and the HBV-DNA positive rates of HBsAg＋HBeAg,HBsAg＋HBeAg＋HBeAb＋HBcAb,HBsAg＋HBeAb,HBsAg＋HBcAb,HBsAg＋HBeAb＋HBcAb,and HBsAg＋HBsAb were 81.82%,72.73%,71.43%,49.21%,37.84%,and 8.33%, respectively. HBsAg and HBeAg infection of HBV-DNA positive pregnant women were higher than those of HBV-DNA negative pregnant women, HBsAb,HbeAb and HBcAb infection of HBV-DNA positive pregnant women were lower than those of HBV-DNA negative pregnant women, and the differences were statistically significant (P<0.05). CONCLUSION There is a certain proportion of HBV infection in pregnant women in Zunyi City, and five quantitative hepatitis B results can reflect HBV replication activity, which is closely associated with HBV-DNA.