Abstract: OBJECTIVE To observe the immune function changes of laryngeal papillomas children with human papilloma virus( HPV) infection, and investigate the relationship between human papilloma virus infection and immune function changes. METHODS A total of 52 cases of laryngeal papilloma tumors children were selected as observation group and 52 cases of healthy children were chosen as control group in Zhoushan Hospital and the First Affiliated Hospital of Zhejiang University from Jan. 2011 to Dec. 2015. HPV and its genotyping in laryngeal papilloma was assayed by fluorescence quantitative polymerase chain reaction, the serum IgM, IgA and IgG concentrations and serum IL-10, IL-6, IL-4, TNF-α and IFN-γ levels were measured, and the peripheral blood CD₄⁺, CD₃⁺, CD₈⁺ content were assayed. RESULTS In 52 cases of children, HPV11 infection was 32 cases, and HPV6 infection was 20 cases. In observation group, onset time was negatively correlated with HPV viral load (r= -0.341, P=0.043).The difference of serum IgM, IgA and IgG concentrations in the two groups was not statistically significant. The CD₄⁺ (29.87±7.83)% and CD₃⁺ (59.89±9.78)% in observation group were lower than those of control group ((36.41±7.67)% and (67.23 ±8.24)%), and the difference was significant (P<0.05).The serum IL-63.07 (2.45-8.34) pg/ml, IL-4(2.59±1.43) pg/ml, TNF-α2.68(1.47-4.37)pg/ml and IFN -γ(4.76 ±2.68)pg/ml levels of observation group were higher than those of control group of2.12 (1.56-2.67) pg/ml, (1.78±0.73)pg/ml, 1.39 (1.06-1.98) pg/ml, and (2.41±0.79)pg/ml, and the difference was statistically significant (P<0.05). CONCLUSION The HPV11 and HPV6 types are children laryngeal papilloma HPV infection types. The time of onset is negatively correlated with HPV viral load.Laryngeal papilloma HPV infection has immune suppression, immune function change is primarily cellular immune changed, and cytokine levels increase.