Abstract: OBJECTIVE To understand the impact of seasonal variation on sterilization quality and hospital infection rates, in order to provide a scientific basis of sterilization monitoring plans for clinical departments. METHODS Air quality, sterilizing equipments、surface of objects, hands of hospital staff, and endosopes, etc. in the same operating room, disinfecting supply division, hemodialysis unit, ICU, and digestive endoscopy room, etc. were sampled using random sampling method every three months. The qualified rates of four sampling items were compared, and the correlations between infection rates and qualified rates were analyzed. RESULTS The environmental overall qualified rate was 92.59%, the hospital disinfection qualified rates of quarterly and various types of items had no significances, and overall sampling qualified rates of different types of items had significances (P＜0.05). ICU hospital infection rates and environmental qualified rates had no correlations with quarterly changes. CONCLUSION Hospital overall monitoring of qualified rate is stable, clinical development of disinfection and sterilization program does not need to consider the factors of seasonal changes, and certain disinfection monitoring should be carried out according to the specific circumstances of the clinical departments.