Abstract: OBJECTIVE To analyze the distribution and drug resistance of pathogenic bacteria in urinary system infection after radical operation of cervical cancer, so as to provide objective evidence for the prevention and control of hospital infection. METHODS A total of 314 patients underwent radical operation of cervical cancer from Mar. 2013 to Aug. 2016 were selected as the research objects. The distribution and drug resistance of pathogenic bacteria in urinary system infection after operation of the patients were observed and analyzed. RESULTS The pathogenic bacteria were detected in urine samples of 112 patients, and the infection rate was 35.7%. Totally 202 strains of bacteria were detected, in which gram-negative bacteria, gram-positive bacteria and fungi accounted for 60.9%, 33.7% and 5.4%,respectively. The main pathogens were Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, and Enterobacter cloacae, accounting for 31.7%, 15.3%, 14.4%, and 10.9%, respectively. In gram-negative bacteria, the resistant rates of Escherichia coli to ampicillin, levofloxacin, tetracycline, sulfamethoxazole/trimethoprim, and ciprofloxacin were higher and more than 85%. In gram-positive bacteria, the resistant rates of K. pneumoniae to ampicillin and nitrofurantion were higher and more than 60%. The resistant rates of Enterobacter cloacae to ampicillin, amoxicillin and cefazolin were 100.0%. No gram-negative pathogens resistant to imipenem were detected. The resistant rates of Enterococcus faecium to erythromycin, tetracycline , rifampicin, and chloramphenicol were higher and more than 50%. The resistant rates of Staphylococcus aureus to penicillin G, levofloxacin, and gentamicin were higher and more than 75%. The resistant rates of Enterococcus faecium to levofloxacin, nitrofurantoin, ampicillin and tetracycline were higher and more than 75%. No gram-positive pathogens resistant to vancomycin were detected. CONCLUSION The distribution and drug resistance of pathogenic bacteria in urinary system infection after radical operation of cervical cancer has certain characteristics. The clinicians should give high attention, close monitoring and effective intervention in order to improve the effects of prevention and control of nosocomial infection.