Abstract: OBJECTIVE To explore the risk factors in patients with urinary tract infection after cystoscopy, so as to provide basis for effective control of urinary tract infection in patients with urinary tract infection. METHODS A total of 96 patients from Jul. 2014 to Jun. 2015 in our hospital after cystoscopy were selected as research subjects. The patient's age, gender, comorbidities, etc. were retrospectively analyzed, and the risk factors of urinary tract infection were analyzed. RESULTS Totally 30 patients were diagnosed with urinary tract infection in 96 patients, with the infection rate of 31.25%. Totally 48 pathogens were detected, including 30 strains of gram-negative bacteria as the main pathogens, accounting for 62.50%, followed by 16 strains of gram-positive bacteria, accounting for 33.33%, and 2 strains of fungi, accounting for 2, 4.17%. The drug sensitivity tests were carried out on three kinds of main pathogens, and the results showed that they had a certain drug resistance to most of the antibiotics. Perfusion more than 6 times, 65 years of age or older, prostate hyperplasia, indwelling catheter, and diabetes and other basic diseases were risk factors of urinary tract infection in patients after cystoscopy. On the basis of single factor analysis, the variables such as the number of perfusion, age, gender, diabetes, prostate hyperplasia and indwelling catheter were statistically analyzed using logistic regression analysis for screening out the independent risk factors. As a result, according to the OR value, they were followed by: age, diabetes, prostate hyperplasia and indwelling catheter. CONCLUSION Preoperative treatment of prostate hyperplasia and diabetes and other basic diseases, and strict control of indications for indwelling catheter and aseptic technique, can effectively reduce urinary tract infections in patients.