Abstract: OBJECTIVE To investigate the pathogenic bacteriological characteristics of elderly patients with diabetic foot and analyze their drug resistance, so as to provide reliable theoretical basis for guiding the clinical use of drugs and avoiding the appearance of resistant strains. METHODS A total of 92 cases of patients with diabetic foot from Feb. 2012 to Dec. 2014 in our hospital were selected. Foot ulcer secretions of patients were collected for bacterial isolation, and the isolates were identified and used for drug sensitivity analysis. RESULTS Of the 92 cases of patients with diabetic foot, 90 pathogens were cultured and isolated, including 47 strains of gram-positive bacteria, 41 strains of gram-negative bacteria, and 2 strains of fungi, accounting for 52.2%, 45.6%, and 2.2%, respectively. Among 47 strains of gram-positive bacteria, 16 strains were multidrug-resistant strains which were resistant to ≥3 drugs, accounting for 34.0%, in which, 14 strains were methicillin-resistant Staphylococcus aureus (MRSA). Staphylococcus susceptibility testing results showed no vancomycin-resistant and linezolid-resistant strains, and highly resistant to penicillin and erythromycin. 41 cases of gram-negative bacteria, the study found A total of 6 strains swere multi-drug resistant strains among 41 stains of gram-negative bacteria, accounting for 14.6%, including 4 strains of Enterobacteriaceae, 2 stains of Pseudomonas aeruginosa. The resistant rates of Enterobacteriaceae to ampicillin and cefazolin were the highest, which were both 91.2%. CONCLUSION In the treatment of patients with diabetic foot infections, it is necessary to give relevant drug susceptibility test and adjust the medication timely and rationally according to the susceptibility test, to reduce the occurrence of drug resistance, promote the healing of patients' foot wounds, improve the prognosis and quality of life of patients, and reduce morbidity and mortality of diabetic foot disease.