Abstract: OBJECTIVE To investigate the risk factors of multi-drug resistant bacteria infection in diabetic foot disease, so as to provide theoretical basis for the development of prevention and treatment measures. METHODS The clinical data of 192 cases of patients with diabetic foot from Jan. 2014 to Jun. 2016 were retrospectively analyzed. The distribution of multi-drug resistant bacteria was analyzed, the clinical data of the two groups of patients were compared, and through the multivariate logistic regression analysis, the risk factors of multi-drug resistant bacteria infection in diabetic foot were explored. RESULTS In this study, 61 cases had multi-drug resistant bacteria infection, and the infection rate was 31.77%. In 93 strains of multi-drug resistant bacteria, 41 strains of Staphylococcus aureus (44.09%) and 20 strains of Pseudomonas aeruginosa (21.51%) were detected. The multi-drug resistant infection rates of the patients with the use of antibiotics, the third generation of cephalosporins, nerve ischemic wound, hospitalization for the same ulcer surface > 2 times, and osteomyelitis were significantly higher than those of no use of antibiotics, no use of the third generation cephalosporins, no nerve ischemic wound, hospitalization for the same ulcer surface less than or equal to 2 times, and no osteomyelitis, and the difference was statistically significant (P<0.05). The multivariate logistic regression analysis showed that the use of antibiotics, nerve ischemic wound, hospitalization for the same ulcer surface more than 2 times and the osteomyelitis were risk factors of multi-drug resistant bacteria infection of diabetic foot (P<0.05). CONCLUSION Diabetic foot had a higher risk of multi-drug resistant bacteria infection. It should develop effective prevention and control measures, according to risk factors for multi-drug resistant bacteria infection in clinic, in order to improve the diagnosis and treatment of the disease, and improve the prognosis of patients.