Abstract: OBJECTIV To explore the influence of trauma sites on distribution and drug resistance of pathogens isolated from limb fracture patients with postoperative infections. METHODS A total of 82 limb fracture patients with postoperative infection who were treated in traumatology department from Jan 2013 to Jul 2016 were retrospectively analyzed; the total infection rate was statistically analyzed, and the distribution and drug resistance of the pathogens isolated from the fracture patients were observed. RESULTS Of 5917 patients with limb fracture, 82 had infections, with the infection rate 1.39%. Totally 98 strains of pathogens were isolated from the 82 patients, including 67 (81.7%) cases of single infection and 15 (18.3%) cases of mixed infection. Among the isolated pathogens, there were 63 strains of gram-positive bacteria, 27 strains of gram-negative bacteria, and 7 strains of fungi. Totally 43 strains of pathogens were isolated from 37 patients with fracture of tibia and fibula, including 31 (72.1%) cases of single infection and 6 (27.9%) cases of mixed infection. Totally 29 strains of pathogens were isolated from 24 patients with femoral facture, including 19 (79.2%) cases of single infection and 5 (20.8%) cases of mixed infection, and the gram-positive bacteria (21 strains) were dominant among the pathogens causing the infection. Totally 25 strains of pathogens were isolated from 21 patients with fracture of ulna and radius, including 17 (81.0%) cases of single infection and 4 (19.0%) cases of mixed infection, and there were 18 strains of gram-positive bacteria and 6 strains of gram-negative bacteria. The Staphylococcus aureus strains were highil susceptible to benzyl ticarcillin and vancomycin, the Staphylococcus epidermidis strains were highly susceptible to vancomycin, Guano cocci strains were highly susceptible to vancomycin; Escherichia coli strains were highly susceptible to imipenem and meropenem, the Enterobacter cloacae strains were highly susceptible to imipenem and meropenem, Pseudomonas aeruginosa strains were highly susceptible to imipenem and meropenem, and Acinetobacter baumannii strains were not susceptible to gentamicin or cefepime but were highly susceptible to imipenem and meropenem. CONCLUSION S.aureus and S.epidermidis are dominant among the pathogens causing the postoperative infections in the patients with limb fracture. It is necessary to conduct the symptomatic treatment of the patients with different sites of trauma and reasonably use antibiotics so as to reduce the incidence of infections in the patients with limb fracture.