Abstract: OBJECTIVE To analyze the related factors, pathogens distribution and drug susceptibility of catheter-related infection in patients treated with maintenance hemodialysis(MHD), so as to provide basis for clinical treatment. METHODS From Jan. 2013 to Dec. 2015, 100 patients treated with MHD were chosen as study objects, and the blood/secretion cultivation results were observed. The related factors, pathogens distribution and drug susceptibility of catheter-related infection in patients were analyzed. The data were analyzed by SPSS16.0 statistical software. The measurement data were expressed as mean ± standard deviation.The χ2 test was used for comparing rate and percentage. The risk factors of infection were analyzed by logistic multivariate analysis,and P <0.05 was considered statistically significant. RESULTS Totally 15 patients (15.00%) were diagnosed with catheter-related infection, which demonstrated significant relativity with age and BMI of patients, and the difference was statistically significant (P＜0.05). Totally 46 strains of bacteria were isolated, including 31 strains (67.39%) of gram-positive bacteria and 15 strains (32.61%) of gram-negative bacteria. Among gram-positive bacteria, Staphylococcus aureus exhibited high drug resistant rates to oxacillin, erythromycin and clindamycin, which were higher than 50.00%, the resistant rate of Staphylococcus epidermidis to oxacillin, sulfamethoxazole / trimethoprim and gentamicin were higher than 30.00%, the resistant rates of Enterococcus to oxacillin, erythromycin and ciprofloxacin were all higher than 60.00%, and they were sensitive to vancomycin, with the drug resistant rates of 0.00%. Gram-negative bacteria were sensitive to ciprofloxacin, levofloxacin, imipenem and meropenem, while were resistant to piperacillin and cefuroxime axetil. Acinetobacter baumannii was resistant to 10 kinds of antimicrobials, while Pseudomonas aeruginosa was resistant to 12 kinds of antimicrobials. CONCLUSION Pathogens isolated from patients with catheter-related infection were multiple drug-resistant, which suggested that the treatment of catheter-related infection should be based on the results of drug susceptibility testing.