Abstract: OBJECTIVE To investigate the prevalence of human herpesvirus 8(HHV-8), human cytomegalovirus (HCMV)and parvovirus B19 (HPVB19) infections among unpaid blood donors, so as to provide data support for transfusion screening strategies. METHODS From Mar. 2015 to Mar. 2016, serum IgM/IgG antibodies of HHV-8, HCMV and HPVB19 were detected in 1 046 cases of unpaid blood donors using enzyme-linked immunosorbent assay (ELISA). RESULTS Among 1046 cases of unpaid blood donors, the positive rates of HHV-8, HCMV, and HPVB19 were 16.6%, 91.3%, and 33.0%, respectively. The positive rates of HHV-8, HCMV and HPVB19 increased with the increase of age, and the difference was statistically significant (P<0.05). The positive rate of HPVB19 infection in females was significantly higher than that in males (P<0.05). The positive rates of HCMV decreased with the increase of education level, and the difference was significant (P<0.05). The positive rates of HHV-8, HCMV and HPVB19 were significantly different among different occupational groups (P<0.05). The positive rates of HHV-8 and HPVB19 were the lowest in students, which were 10.7% and 25.9%, and the lowest rate of HCMV infection was 87.5% in civil servants and enterprises. The positive rates of HHV-8, HCMV and HPVB19 had no significant difference among different blood types. CONCLUSION There are different degrees of infections of HHV-8, CMV and HPV B19 in this region. When conditions permit, the 3 viruses should be detected for blood transfusion in key populations.