Abstract: OBJECTIVE To understand the transitivity of blaNDM-1 plasmid, plasmid replicon typing, and genetic environment in Enterobacter aerogenes isolates. METHODS The E.aerogenes isolates were recruited as the study objects, then the transitivity of the plasmids was identified via plasmid binding assay, the plasmid replicon typing was carried out by using PCR, the DNA downstream and upstream around blaNDM-1 were sequenced by means of DNA walking method, and the genomic sequences were aligned by using BLASTN and BALSTP and were annotated with the use of Vector NTI 11.5.1. RESULTS The E.aerogenes isolates were positive for the plasmid binding assay, the plasmid rellicon was type IncA/C. The blaNDM-1 gene localized between ISAba14 and IS91. Class 1 integron, followed by Tn3 transposon, was identified in the upstream of the blaNDM-1, and the class 1 integron bore an unique resistance gene cassette consisting of large mosaic sequences. CONCLUSION The pHN-NDM0711 bears blaNDM-1 gene in an IncA/C plasmid, and the resistance gene cassette derives from gene recombination under the pressure of selection of different antibiotics. The reasonable use of clinical, industrial, and agricultural antibiotics may prevent such species of pathogen from emerging.