Abstract: OBJECTIVE To explore the pathogenic bacteria distribution and drug resistance in patients with diabetic foot, so as to provide some guidance for its clinical treatment. METHODS The clinical data of 287 patients with diabetic foot from Jul. 2006 to Jun. 2016 were retrospectively analyzed. The pathogenic bacteria distribution and drug resistance of foot infection in diabetic patients were analyzed. RESULTS A total of 324 strains of bacteria were isolated from 287 cases of patients with diabetic foot, including 149 strains of gram-positive bacteria accounted for 46%, 153 strains of gram-negative bacteria accounted for 47.2%, and 22 strains of fungi accounting for 6.8%. Gram-positive bacteria were mainly Staphylococcus aureus, Enterococcus faecalis and coagulase negative Staphylococci, gram-negative bacteria were mainly Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa. With the increase of Wagner grade, the main pathogenic bacteria gradually changed from gram-positive bacteria to gram-negative bacteria. Over the past 10 years, Staphylococcus was resistant to clindamycin, ampicillin, and penicillin, and was sensitive to amikacin, linezolid, teicoplanin, and vancomycin. The resistant rate of Staphylococcus to amoxicillin/clavulanic acid was 47.8% in the past 5 years, which was significantly higher than 12.5% in the previous 5 years, and the difference was statistically significant (P<0.05). In recent 10 years, Enterobacteriaceae was highly resistant to ampicillin and was sensitive to imipenem, piperacillin / tazobactam and meropenem. In recent 5 years, the resistant rate of Enterobacteriaceae to cefotaxime was 45.5%, which was significantly higher than 28.6% of the previous 5 years, and the difference was statistically significant (P<0.05). CONCLUSION .Gram-positive bacteria and gram-negative bacteria were almost the same in foot infection in patients with diabetes mellitus in the past 10 years, and the status of drug resistance was common. It should rationally choose antibiotics based on pathogen distribution and drug resistance, to avoid the emergency of drug-resistant bacteria