Abstract: OBJECTIVE To discuss the correlation between pathogenic bacteria on the surface of nurse workstation and hand hygiene of nursing staff, so as to provide guidances for the prevention and control of nosocomial infection. METHODS A total of 25 nurse workstations and 50 nursing staff from different departments in Jun. 2016 were selected. The computer keyboard, mouse and the front part of the computer desktop were sampled separately before and after disinfection, and the pathogenic bacteria were cultured and identified. The hand hygiene compliance of nursing staff was randomly observed, and their attitudes to hand hygiene were investigated. RESULTS Totally all 75 specimens from the surface of 25 nurse workstations were detected of pathogenic bacteria before disinfection, the detection rate of Staphylococcus aureus was 54.67%, and the detection rates of Bacillus subtilis, Acinetobacter, Micrococcus, Escherichia coli, Proteus, Pseudomonas aeruginosa, Streptococcus haemolyticus and other pathogenic bacteria were 46.67%, 36.00%, 29.33%, 21.33%, 10.67%, 6.67%, 5.33%, and 5.33%, respectively. The detection bacteria rate of keyboard, mouse and desktop after disinfection were significantly lower than those before disinfection, and the difference was statistically significant(P<0.05). It was observed that the nursing staff should wash their hands 367 times, but the actual number was 62 times, and the compliance was 16.89%. The hand hygiene compliance of nursing staff working for ≥ 20 years, with intermediate or senior title, and with education level of bachelor or higher were higher than those with working period ＜ 20 years, junior title, and with education level of junior college or lower, and the difference was statistically significant(P<0.05). The scores of attitude to hand hygiene of nursing staff with working period ≥ 20 years and with intermediate or senior title were higher than those with working period＜ 20 years and junior title, and the difference was statistically significant(P<0.05). CONCLUSION The pathogenic bacteria on the surface of nurse workstation was serious, and was closely related with hand hygiene compliance and the attitude to hand hygiene of nursing staff. It should improve the hand hygiene compliance and attitude of nursing staff, periodically disinfect the workstation, and reduce pollution.