Abstract: OBJECTIV To observe the distribution and clinical drug resistance of pathogens isolated from patients with infective endocarditis in recent 10 years so as to provide reliable basis for reasonable clinical use of antibiotics. METHODS By means of retrospective analysis, a total of 286 patients who were diagnosed with infective endocarditis in department of cardiology of the hospital from Jan 2008 to Jan 2016 were recruited as the study objects, the related information was recorded in detail, the pathogens that were cultured from blood were identified by using VITEK2 Compact and matched identification card, and the drug susceptibility testing was performed with the use of drug susceptibility card. RESULTS Of the 286 patients with infective endocarditis, 208 were positive for blood culture, with the positive rate 72.7%. Totally 288 strains of pathogens were isolated, including 156 (54.2%) strains of gram-positive bacteria, 92 (31.9%) strains of gram-negative bacteria, and 40 (13.9%) strains of fungi. The drug susceptibility rate of the gram-positive bacteria was the highest to linezolid, followed by vancomycin and moxifloxacin; the drug resistance rate was the highest to penicillin, followed by oxacillin. The gram-negative bacteria were the most susceptible to imipenem, followed by meropenem. The fungi were most susceptible to fluconazole, followed by voriconazole. CONCLUSION The proportions of the pathogens isolated from the patients with infective endocarditis have changed, the proportion of the gram-positive bacteria has reduced, however, the proportions of the gram-negative bacteria and fungi have increased. In clinical practice, it is necessary to identify the pathogens and understand the status of drug susceptibility so as to conduct the targeted treatment.