Abstract: OBJECTIVE To investigate the prevalence and relationship of common resistant genes to carbapenem-resistant Klebsiella pneumoniae (CRKP) in our hospital. METHODS Twenty strains of CRKP isolated from our hospital from Jan. 2014 to Jan. 2016 were collected and analyzed by PCR for the detection of 40 β-lactamase genes and 11 markers of mobile genetic elements (MGEs), and 20 kinds of aminoglycoside-acquired drug resistant genes were detected simultaneously. The results were analyzed by cluster analysis to find out their genetic relationship. RESULTS Twenty strains of CRKP were found to be resistant to β-lactam drugs (imipenem, meropenem, cefotaxime, cefepime, cefepime, ampicillin/sulbactam, piperacillin/tazobactam), aminoglycoside drugs (gentamicin, amikacin), and quinolones (norfloxacin, ciprofloxacin, levofloxacin). BlaTEM and blaKPC β-lactamase genes were detected in 20 strains of CRKP, and aminoglycoside drug-resistant genes were also detected in each strain, but different genes had different positive rates. MGEs were also detected in each strain, but the positive rates of intⅠ1 and IS903 were different. The cluster analysis showed that 20 strains of CRKP showed obvious aggregation (group A) and could be divided into A-A group and A-B group. CONCLUSION There are some homologies between CRKP strains in our hospital. The major prevalent genotypes are blaTEM and blaKPC. The prevalent markers of MGEs are trbC, ISEcp1, IS26, and ISKpn6, and it needs to strengthen hospital infection management.