Abstract: OBJECTIV To explore the influence of standard filling out the first page of medical record on prevalence rate of nosocomial infections so as to provide guidance for control of the nosocomial infections. METHODS A totalof 2748 patients who were treated in hospitals from Mar 2012 to Feb 2016 were enrolled in the study and divided into two groups according to the admission time, 1469 patients who were admitted from Mar 2014 to Feb 2016 were assigned as the standard group, and 1279 patients who were admitted from Mar 2012 to Feb 2014 were set as the non-standard group; meanwhile, 416 health care workers who were on duty were enrolled in the study, 214 health care workers were assigned as the standard group (from Mar 2014 to Feb 2016), and 202 health care workers were set as the non-standard group (from Mar 2013 to Feb 2014). The level of standard medical record, status of filling out the first page of medical record, incidence of nosocomial infection in patients, and rates of complaint, dispute, and satisfaction were observed and compared between the two groups of health care workers. RESULTS There was significant difference in the awareness of standardization of medical record of the health care workers, related knowledge of standardization of medical record, and degree of standardization of medical record between the standard group and the non-standard group (P＜0.05).Among the 1469 medical records in the standard group, 19 (1.29%) cases were missing, 22 (1.50%) were inaccurate, and 12 (0.82%) cases were wrong. Among the 1279 medical records in the non-standard group, 102 (7.97%) were missing, 168 (13.14%) were inaccurate, and 29 (2.27%) cases were wrong. The incidence of nosocomial infection was 0.75% among the 1469 patients in the standard group, and the incidence of nosocomial infections was 5.94% among the 1279 patients in the non-standard group. The rates of complaint, dispute, and satisfaction of the standard group were 0.34%, 0.27%, and 97.00%, respectively; the rates of complaint, dispute, and satisfaction of the non-standard group were 3.05%, 5.94%, and 83.03%, respectively. CONCLUSION The standard filling out of the first page of medical record may intensify the awareness of standardization of medical record, reduce the problems of missing, wrong filling out the medical records, and prevent the nosocomial infections; it is worthy to be promoted in the hospital.