Abstract: OBJECTIV To explore the bacterial culture of secretions of patients with chronic suppurative otitis media and analyze the result of drug susceptibility testing. METHODS A total of 80 patients with chronic suppurative otitis media who were hospitalized for treatment from Oct 2013 to Oct 2015 were enrolled in the study. The conventional bacterial culture was carried out for ear canal secretions that were obtained from the patients, the isolated bacteria were identified, and the result of drug susceptibility testing was analyzed. RESULTS The pathogens were isolated from the secretions specimens of 68 of 80 patients, and the isolation rate of pathogens was 85.00%. Totally 74 strains of pathogens were cultured, including 32 (43.24%) strains of gram-positive bacteria, 32 (43.24%) strains of gram-negative bacteria, and 10 (13.51%) strains of fungi. The drug resistance rate of Staphylococcus aureus and Staphylococcus epidermidis strains to penicillin was more than 80%, the drug resistance rate to clindamycin and erythromycin was more than 50%. The drug resistance rate of Pseudomonas aeruginosa and Klebsiella pneumoniae strains to ampicillin, cefazolin, ceftriaxone, cefotetan, nitrofurantoin, ampicillin-sulbactam, and sulfamethoxazole was more than 90%, and the drug resistance rate to cefotaxime was more than 60%. The gram-positive bacteria and gram-negative bacteria were highly susceptible to the commonly used antibiotics such as levofloxacin. Candida albicans and Aspergillus strains showed certain resistance to ketoconazole, econazole, and miconazole, however, the strains were highly susceptible to nystatin, amphotericin, and 5-fluorocytosine. CONCLUSION S.aureus, S.epidermidis, and P.aeruginosa are the common species of pathogens that are isolated from the secretions specimens obtained from the patients with chronic suppurative otitis media. It is necessary for the hospital to reasonably use antibiotics based on the results of the bacterial culture and drug susceptibility testing.