Abstract: OBJECTIV To observe the vaginal secretions and the infections of chlamydia and mycoplasma of female patients,so as to provide the basis for clinical treatment. METHODS selected total of 1200 cases of newly diagnosed female patients from Jan. to Dec. 2015 in the hospital were selected for the study. The detection results of vaginal secretions and chlamydia and mycoplasma were collected. RESULTS In the 1200 cases of vaginal secretions, fungal vaginitis were 143 cases, with the infection rate of 11.9%, bacterial vaginosis were 94 cases, with the infection rate of 7.8%, and trichomonas vaginitis were 11 cases, with the infection rate of 0.9%. There was no significant difference in the infection rates of mycotic vaginitis, bacterial vaginosis and trichomonas vaginitis among different age groups. The incidence of mycotic vaginitis, bacterial vaginosis and trichomonas vaginitis in patients with Ⅲ-Ⅳ degree of vaginal cleanliness were significantly higher than those with Ⅰ-Ⅱ degree of vaginal cleanliness (P<0.05). In 1,200 cases of patients, the cervical chlamydia infection were 139 cases, with the infection rate of 11.6%, and the mycoplasma infection were 449 cases, with the infection rate of 37.4%. The detection rate of chlamydia and mycoplasma in different age groups was significant (P<0.05). The detection rates of cervical chlamydia in 25-34 years old and 35-44 years old were higher, which were 15.2% and 17.0% %, and the detection rates of mycoplasma in 25-34 years old and 35-44 years old were also higher, which were 50.2% and 49.5%. CONCLUSION The infection rates of female fungal vaginitis and bacterial vaginosis are higher. The occurrence of vaginitis is closely related to vaginal cleanliness. Chlamydia and mycoplasma are common pathogens of cervix, and the detection rate in the 35-44 year-old is high.