Abstract: OBJECTIV To investigate the prevalence of nosocomial infections in high-risk pregnant women and analyze the related influencing factors so as to provide guidance for clinical diagnosis and treatment. METHODS A total of 1000 pregnant women who were treated in department of gynecology and obstetrics from Jan 2014 to Dec 2015 were recruited as the study objects; 586 high-risk pregnant women were assigned as the high-risk group, and 414 normal pregnant women were set as the control group. The clinical data of all the pregnant women were collected. RESULTS The incidence rate of nosocomial infections was higher in the high-risk group than in the control group (P<0.05). Of 23 high-risk pregnant women with the nosocomial infections, 11 (47.8%) had respiratory tract infection, 7 (30.4%) had genital tract infection, 4 (17.4%) had incision infection, and 1 (4.3%) had urinary tract infection. Totally 20 strains of pathogens were isolated from the 23 high-risk pregnant women with nosocomial infections, including 11 (55.0%) strains of gram-positive bacteria, 8 (40.0%) strains of gram-negative bacteria, and 1 (5.0%) strain of fungus. The incidence rate of nosocomial infections was lower in the pregnant women who received standard prenatal examination, had urban residential registration, did not receive invasive operations, stayed in hospital for less than 7 days, or underwent the vaginal delivery than in the pregnant women who did not receive standard prenatal examination, had rural residential registration, receive invasive operations, stayed in hospital for no less than 7 days, or did not receive the vaginal delivery (P<0.05). The invasive operation and delivery modes were the related independent factors for the nosocomial infections in the high-risk pregnant women. CONCLUSION The prevalence rate of nosocomial infections is high in the high-risk pregnant women; Staphylococcus aureus and Escherichia coli are dominant among the pathogens causing the nosocomial infections. The invasive operation and delivery mode are the independent influencing factors for the nosocomial infections in the high-risk factors.